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The histology and histomorphometric analyses suggested that the pore size of a scaffold could directly modulate the pattern and degree of neovascularization. Figure 8 shows the mechanisms proposed to explain these observations on the development of neovasculatures in inverse opal scaffolds with different pore sizes. Cameliet P, Conway EM. Lastly, in addition to endothelial cells and pericytes, the migration of non-vascular cells e. PLGA inverse opal scaffolds were fabricated according to our previous reports. Figure S1 was produced as previously described [ 31 ]. Cell proliferation assay Cell proliferation was determined by the MTT uptake method.

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Figure 4B shows plots of the blood vessel-to-tissue area ratio as a function of pore size at 2 and 4 weeks post implantation.

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After the initial infiltration of blood vessels from the top surface of the scaffolds, the pore size should then play an important role when these vessels further branch and penetrate deeper. Subcutaneous pockets were created lateral to both incisions by separating the subcutaneous facial plane using blunt dissection. Tissue engineering has emerged as a promising strategy for the regeneration of tissues and organs damaged by injuries or diseases. DAPI was used to stain the nuclei. Histological assessment and histomorphometric analyses Following explantation, the PLGA scaffolds were fixed in 3.

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